fluorescent probe jc 1  (Beyotime)

Journal: Bioactive Materials

Article Title: Injectable microgels carrying engineered biomimetic nanoparticles for osteoarthritis therapy via dual-targeted senescent chondrocyte clearance and endogenous repair promotion

doi: 10.1016/j.bioactmat.2025.11.038

Figure Lengend Snippet: Preparation and characterization of ANCM@SHM. (a) Schematic diagram of NCM preparation. (b) Representative TEM images of native CM and NCM. Bar: 50 nm. (c–d) Zeta potential (n = 3), particle size (n = 3), and PDI (n = 3) of native CM and NCM measured by DLS. (e) Representative TEM images of Lipo, A-lipo, and ANCM. Bar: 50 nm. (f–g) Zeta potential (n = 3), particle size (n = 3), and PDI (n = 3) of Lipo, A-lipo, and ANCM measured by DLS. (h) Representative confocal fluorescence micrographs showing red fluorescently labeled A-lipo and green fluorescently labeled NCM fusing into gold fluorescent ANCM. Bar: 10 μm. (i) Quantification of NKG2D on the surface of CM, NCM, and ANCM nanoparticles by flow cytometry. (j–k) Encapsulation efficiency (EE) (n = 3) and loading efficiency (LE) (n = 3) of ABT263. (l) Representative SEM image of ANCM@SHM. Bar: 100 μm. (m) Representative fluorescence image of PKH67-ANCM@SHM captured by confocal microscope. Bar: 100 μm.

Article Snippet: Subsequently, cells were incubated in the dark at room temperature for 20 min. Mitochondrial membrane potential was assessed employing the JC-1 fluorescent probe (Beyotime, China) following manufacturer's protocols.

Techniques: Zeta Potential Analyzer, Fluorescence, Labeling, Flow Cytometry, Encapsulation, Microscopy

Journal: Journal of Inflammation Research

Article Title: Integrated Machine Learning and Multi-Omics Analysis Identifies Mitophagy-Related Core Genes and Mechanisms in Non-Alcoholic Fatty Liver Disease

doi: 10.2147/JIR.S575586

Figure Lengend Snippet: Experimental validation. ( A ) qRT-PCR results for core gene mRNA expression (**P < 0.01, ***P < 0.001). ( B ) Western Blot bands for core proteins. ( C ) Oil Red O staining of control and NAFLD model HepG2 cells. ( D ) JC-1 fluorescence staining (red: aggregates, high potential; green: monomers, low potential).

Article Snippet: The primary antibodies used were as follows: Anti-IGF1 Rabbit Polyclonal Antibody (IGF1, Catalog No. A11985; ABclonal Technology, China), Anti-MYH11 Rabbit Monoclonal Antibody (MYH11, Clone EPR5335; Abcam, Cambridge, UK), Anti-HYOU1 Rabbit Polyclonal Antibody (HYOU1, Catalog No. A1042; ABclonal Technology, Wuhan, China), Anti-SPATA18 Rabbit Polyclonal Antibody (SPATA18, Catalog No. A09906-1; ABclonal Technology, Wuhan, China), Anti-SCD (Stearoyl-CoA Desaturase) Rabbit Monoclonal Antibody (SCD, Catalog No. ab236868; Abcam, Cambridge, UK), and Anti-β-Actin Mouse Monoclonal Antibody (β-Actin, Catalog No. AC026; ABclonal Technology, Wuhan, China).Sodium palmitate (PA) and sodium oleate (OA) were obtained as a ready-made mixture (PA 6 mmol/L, OA 12 mmol/L; Catalog No. KC006) from Xi’an Kunchuang Technology Development Co., Ltd. (China).The mitochondrial membrane potential fluorescent probe JC-1 (Catalog No. C2006) was supplied by Beyotime Biotechnology (China).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Expressing, Western Blot, Staining, Control, Fluorescence